α cxcl13  (R&D Systems)

Journal: Cell Genomics

Article Title: CXCR4 orchestrates the TOX-programmed exhausted phenotype of CD8 + T cells via JAK2/STAT3 pathway

doi: 10.1016/j.xgen.2024.100659

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Article Snippet: Rat Anti-Mouse CXCL13 Monoclonal Antibody, APC , Thermo Fisher Scientific , Cat# 17-7981-82; RRID:AB_2762702.

Techniques: Recombinant, Lysis, Activation Assay, Staining, Magnetic Beads, Control, Amplification, Immunohistochemistry, Cell Isolation, RNA Extraction, Sonication, Chromatin Immunoprecipitation, Software

Journal: JCI insight

Article Title: The homing of exogenous hair follicle mesenchymal stem cells into hair follicle niches.

doi: 10.1172/jci.insight.173549

Figure Lengend Snippet: Figure 8. Chemokine mediates the homing of exogenous hfMSCs into follicle niches. (A) RT-qPCR experiments showed the upregulated chemokines after depilation of NOD/SCID dorsal skin (n = 3). (B) Transwell chemotaxis assays were performed to detect the migratory response of DPCs and DSCs to chemokines at different concentrations (namely, control: 0 ng/mL; low: 5 ng/mL; medium: 50 ng/mL; and high: 500 ng/mL) (n = 5). (C) Wound healing assays were performed to detect the chemotaxis effects of CXCL2, CXCL13, and CXCL16 on DPCs and DSCs (concentrations are the same as in B) (n = 3). One-way ANOVA followed by Bonferroni’s post hoc test. Data reported as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: For the neutralizing CXCL13 study, 50 μg/mL CXCL13 neutralizing Ab (R&D Systems, Bio-Techne; catalog AF470) was mixed with 2 × 106 DPCs/ DSCs, and the mixture was intradermally injected into depilated back skin of adult NOD/SCID mice.

Techniques: Quantitative RT-PCR, Chemotaxis Assay, Control

Journal: JCI insight

Article Title: The homing of exogenous hair follicle mesenchymal stem cells into hair follicle niches.

doi: 10.1172/jci.insight.173549

Figure Lengend Snippet: Figure 9. Chemokine receptor mediates the homing of exogenous hfMSCs into follicle niches. (A) Dorsal skin sections were harvested 1, 2, 4, and 6 days after depilation. (The control group comprised a section of shaved dorsal skin.) Immunostaining showed the expression of CXCL13, CXCL16, and CXCL2 (representative of 3 experiments). (B and C) RT-qPCR experiments showed the expression of receptors related to upregulated chemokines in low-passage and high-passage DPCs/DSCs (n = 3). Two-tailed Student’s t test. Data reported as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. (D) Venn diagram shows the intersection of the downregulated chemokine receptors in high-passage DPCs/DSCs. (E) Immunostaining shows the expression of CXCR5 in low-passage and high-passage DPCs/DSCs (representative of 3 experiments). Scale bars: 100 μm in A and E.

Article Snippet: For the neutralizing CXCL13 study, 50 μg/mL CXCL13 neutralizing Ab (R&D Systems, Bio-Techne; catalog AF470) was mixed with 2 × 106 DPCs/ DSCs, and the mixture was intradermally injected into depilated back skin of adult NOD/SCID mice.

Techniques: Control, Immunostaining, Expressing, Quantitative RT-PCR, Two Tailed Test

Journal: JCI insight

Article Title: The homing of exogenous hair follicle mesenchymal stem cells into hair follicle niches.

doi: 10.1172/jci.insight.173549

Figure Lengend Snippet: Figure 10. The CXCL13/CXCR5 axis mediates the homing of exogenous hfMSCs into follicle niches. (A) RT-qPCR experiments show the mRNA expression of CXCR5 in DPCs/DSCs after transfection with lentiviral vector expressing CXCR5 shRNA (n = 3). (B) Immunostaining shows the protein expression of CXCR5 in DPCs/DSCs after transfection with lentiviral vector expressing CXCR5 shRNA4 (representative of 3 experiments). (C) DPCs/DSCs transfected with lentiviral vector expressing CXCR5-shRNA4 were injected into the dorsal skin of depilated NOD/SCID mice. (D) DPCs/DSCs mixed with CXCL13 neutralizing Ab were injected into the dorsal skin of depilated NOD/SCID mice. HFs containing exogenous DPCs (green; arrow) or DSCs (green; arrow) were immunos- tained for alkaline phosphatase (ALP) (red) (C and D). (E and F) Number of HFs containing EGFP+ transplanted cells and number of homing EGFP+ cells per ×100 original magnification field of view, as shown in C and D (n = 7 skin sections from 4 mice per group). Statistical comparisons were performed using 1-way ANOVA followed by Bonferroni’s post hoc test (A) and 2-tailed Student’s t test (E and F). Scale bars: 100 μm. Data are reported as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: For the neutralizing CXCL13 study, 50 μg/mL CXCL13 neutralizing Ab (R&D Systems, Bio-Techne; catalog AF470) was mixed with 2 × 106 DPCs/ DSCs, and the mixture was intradermally injected into depilated back skin of adult NOD/SCID mice.

Techniques: Quantitative RT-PCR, Expressing, Transfection, Plasmid Preparation, shRNA, Immunostaining, Injection